3CEQ

The TPR domain of Human Kinesin Light Chain 2 (hKLC2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Collection date	2007-07-07
Wavelength(s)	.979
Spacegroup name	P 21 21 21
Unit cell lengths	70.422, 99.940, 103.106
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.750
R-factor	0.236
Rwork	0.234
R-free	0.27100
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.845
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.690
High resolution limit [Å]	2.600	5.600	2.600
Rmerge	0.144	0.070	0.583
Number of reflections	21563
<I/σ(I)>	16.5
Completeness [%]	94.3	99.8	68.8
Redundancy	13.3	16	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	293	1.46 M Ammonium Sulfate, 0.2 M Sodium Acetate, 0.1 M Bis-Tris pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K