3CEJ

Human glycogen phosphorylase (tense state) in complex with the allosteric inhibitor AVE2865

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Spacegroup name	P 31
Unit cell lengths	123.270, 123.270, 121.880
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.470 - 3.300
R-factor	0.181
Rwork	0.176
R-free	0.27100
RMSD bond length	0.009
RMSD bond angle	1.259
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	3.300
Number of reflections	25212
Completeness [%]	80.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5		VAPOR DIFFUSION, HANGING DROP, 7 mg/ml in 20 mM BES, 1 mM EDTA, 0.5 mM DTT, pH 6.5, 50 mM NBG, 2.5 mM AVF (AVE2865)