3CED
Crystal structure of the C-terminal NIL domain of an ABC transporter protein homologue from Staphylococcus aureus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793, 0.9795 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 132.550, 132.550, 132.083 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.400 - 2.150 |
| R-factor | 0.186 |
| Rwork | 0.184 |
| R-free | 0.21500 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.303 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.087 | 0.463 |
| Number of reflections | 32344 | |
| <I/σ(I)> | 8.2 | 2.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 16.3 | 16.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 289 | 20% 1,4-Butanediol, 0.1M Acetate pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
