3CE1
Crystal Structure of the Cu/Zn Superoxide Dismutase from Cryptococcus liquefaciens Strain N6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-13 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 35.854, 111.768, 70.456 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.200 |
R-factor | 0.15 |
Rwork | 0.149 |
R-free | 0.16600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f1g |
RMSD bond length | 0.010 |
RMSD bond angle | 1.362 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.530 | 1.240 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.065 | 0.459 |
Number of reflections | 44741 | |
<I/σ(I)> | 31.3 | 3.5 |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 7 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 30% PEG 4000, 0.2M sodium acetate, 0.1M Tris-Cl , pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |