3CDY
AL-09 H87Y, immunoglobulin light chain variable domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-01 |
| Detector | SBC-3 |
| Wavelength(s) | 0.979508 |
| Spacegroup name | P 61 |
| Unit cell lengths | 73.971, 73.971, 95.070 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 64.020 - 2.430 |
| R-factor | 0.19293 |
| Rwork | 0.189 |
| R-free | 0.26265 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q20 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.789 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.061 | 2.520 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Number of reflections | 10539 | |
| <I/σ(I)> | 39.38 | 7.86 |
| Completeness [%] | 94.1 | 91.5 |
| Redundancy | 11.2 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 295 | 30% w/v polyethylene glycol 4000 and 0.2 M Li2SO4 in 0.1 M Tris buffer, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
