3CDN
Crystal structure of a pheromone binding protein from Apis mellifera soaked at pH 4.0
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-29 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 0.931 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 79.146, 83.530, 47.361 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.000 |
R-factor | 0.17382 |
Rwork | 0.171 |
R-free | 0.20315 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3bjh |
RMSD bond length | 0.013 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | REFMAC (5.2.0019) |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.450 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.069 | 0.460 |
Number of reflections | 10923 | |
<I/σ(I)> | 15.3 | 3.4 |
Completeness [%] | 99.8 | 100 |
Redundancy | 5 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 1.9M ammonium sulfate, 0.1M sodium citrate, pH5.5, crystal was soaked in the same condition at pH4.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |