3CDH
Crystal structure of the MarR family transcriptional regulator SPO1453 from Silicibacter pomeroyi DSS-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-21 |
| Detector | APS-1 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 124.140, 124.140, 140.186 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.370 - 2.690 |
| R-factor | 0.20154 |
| Rwork | 0.200 |
| R-free | 0.22748 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.623 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.370 | 2.800 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.115 | 0.835 |
| Number of reflections | 18036 | |
| <I/σ(I)> | 7.2 | 2.6 |
| Completeness [%] | 99.5 | 94.2 |
| Redundancy | 11.9 | 12.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 2.0M Ammonium sulfate, 0.1M Citrate pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
