3CD6
Co-cystal of large Ribosomal Subunit mutant G2616A with CC-Puromycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 212.805, 299.997, 576.002 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.950 - 2.750 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.850 |
| High resolution limit [Å] | 2.750 | 5.920 | 2.750 |
| Rmerge | 0.094 | 0.038 | |
| Number of reflections | 468902 | ||
| <I/σ(I)> | 13.2 | ||
| Completeness [%] | 99.1 | 98.6 | 93.3 |
| Redundancy | 5.3 | 5.3 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sitting drop | 5.8 | 292 | PEG6000, KCL, NH4CL, MGCl2, pH 5.80, sitting drop, temperature 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG6000 | ||
| 2 | 1 | 1 | KCL | ||
| 3 | 1 | 1 | NH4CL | ||
| 4 | 1 | 1 | MGCl2 | ||
| 5 | 1 | 2 | PEG6000 | ||
| 6 | 1 | 2 | KCL | ||
| 7 | 1 | 2 | NH4CL | ||
| 8 | 1 | 2 | MGCl2 |
