3CC2
The Refined Crystal Structure of the Haloarcula Marismortui Large Ribosomal Subunit at 2.4 Angstrom Resolution with rrnA Sequence for the 23S rRNA and Genome-derived Sequences for r-Proteins
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-02-25 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 211.646, 299.672, 573.773 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.950 - 2.400 |
R-factor | 0.199 |
Rwork | 0.199 |
R-free | 0.23100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.100 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 90.000 |
High resolution limit [Å] | 2.400 |
Rmerge | 0.086 |
Number of reflections | 666819 |
<I/σ(I)> | 26.6 |
Completeness [%] | 95.6 |
Redundancy | 9.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | sitting drop | 5.8 | 292 | PEG6000, KCL, NH4CL, MGCl2, pH 5.80, sitting drop, temperature 292K |
1 | sitting drop | 5.8 | 292 | PEG6000, KCL, NH4CL, MGCl2, pH 5.80, sitting drop, temperature 292K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG6000 | ||
2 | 1 | 1 | KCL | ||
3 | 1 | 1 | NH4CL | ||
4 | 1 | 1 | MGCl2 | ||
5 | 1 | 2 | PEG6000 | ||
6 | 1 | 2 | KCL | ||
7 | 1 | 2 | NH4CL | ||
8 | 1 | 2 | MGCl2 |