3CBH

THREE-DIMENSIONAL STRUCTURE OF CELLOBIOHYDROLASE FROM TRICHODERMA REESEI

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	49.100, 75.800, 92.900
Unit cell angles	90.00, 103.20, 90.00

Refinement procedure

Resolution	8.000 - 2.000
Rwork	0.155
RMSD bond length	0.011 *
RMSD bond angle	2.700 *
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	2.800 *
High resolution limit [Å]	0.550 *
Rmerge	0.076 *
Total number of observations	99337 *
Number of reflections	32934 *
Completeness [%]	97.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	PEG6000	10 (%)
3	1	drop	MES	20 (mM)
4	1	reservoir	PEG6000	20 (%)
5	1	reservoir	MES	20 (mM)