3CAB
Crystal structure of a pheromone binding protein from Apis mellifera soaked at pH 7.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 75.478, 86.185, 49.557 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.950 |
| R-factor | 0.17848 |
| Rwork | 0.175 |
| R-free | 0.21714 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3bjh |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.362 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC (5.2.0019) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.800 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.060 | 0.413 |
| Number of reflections | 12126 | |
| <I/σ(I)> | 21.8 | 4.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.9 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1.7M ammonium sulfate, 0.1M sodium citrate, pH5.5, crystal was soaked in same conditions at pH7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
