Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1996-09 |
| Detector | ADSC QUANTUM |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.120, 77.730, 79.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.28000 |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | 2CAA |
| RMSD bond length | 0.022 |
| RMSD bond angle | 25.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.101 * | |
| Total number of observations | 63094 * | |
| Number of reflections | 15560 | |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 83.7 | 85.6 |
| Redundancy | 4.05 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 | Lukacs, C.M., (1996) Nat.Struct.Biol., 3, 888. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2 (mg/ml) | |
| 2 | 1 | drop | mPEG5000 | 3.3 (%) | |
| 3 | 1 | drop | magnesium acetate | 0.07 (M) | |
| 4 | 1 | drop | sodium citrate | 0.03 (M) | |
| 5 | 1 | reservoir | mPEG5000 | 10 (%) | |
| 6 | 1 | reservoir | magnesium acetate | 0.2 (M) | |
| 7 | 1 | reservoir | sodium citrate | 0.1 (M) |
