3C9H
Crystal structure of the substrate binding protein of the ABC transporter from Agrobacterium tumefaciens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.313, 86.947, 109.642 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.600 - 1.900 |
| R-factor | 0.17539 |
| Rwork | 0.173 |
| R-free | 0.22817 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.371 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.200 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.112 | 0.731 |
| Number of reflections | 54136 | |
| <I/σ(I)> | 19.78 | 1.57 |
| Completeness [%] | 99.3 | 97.77 |
| Redundancy | 8.7 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 289 | 0.1M Sodium citrate, 0.2M Ammonium acetate, 30% PEG 4000, 10mM ATP, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
