3C9G
Crystal structure of uncharacterized UPF0201 protein AF_135
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-03 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.986, 79.986, 95.374 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.450 - 2.300 |
| R-factor | 0.269 |
| Rwork | 0.269 |
| R-free | 0.32400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nwu |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.290 |
| Rmerge | 0.098 |
| Number of reflections | 25121 |
| <I/σ(I)> | 6.5 |
| Completeness [%] | 99.3 |
| Redundancy | 13.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 298 | 0.1 M Sodium citrate pH 5.2, 30% PEG 4000, 0.1M Ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
