3C9B
Crystal structure of SeMet Vps75
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2008-01-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.203, 88.937, 94.259 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.420 |
| R-factor | 0.214 |
| Rwork | 0.212 |
| R-free | 0.25600 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.902 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.550 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.217 | 0.150 |
| Number of reflections | 26011 | |
| <I/σ(I)> | 20.6 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 22.5 | 18.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.2 M K Formate, 25% PEG3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
