3C97
Crystal structure of the response regulator receiver domain of a signal transduction histidine kinase from Aspergillus oryzae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-14 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 48.501, 48.501, 91.669 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.248 |
| Rwork | 0.246 |
| R-free | 0.27100 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.578 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.003 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.106 | 0.581 |
| Number of reflections | 14328 | |
| <I/σ(I)> | 10.8 | 2.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.2 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 294 | 100mM MES pH 6.5, 25% PEG 1000, VAPOR DIFFUSION, temperature 294K |
