3C94
ExoI/SSB-Ct complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2006-08-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.151, 92.191, 91.277 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.201 |
| Rwork | 0.198 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fxx |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.052 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.079 | 0.383 |
| Number of reflections | 16073 | |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 98.0 | 88.2 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 18-27% PEG4000, 0.1-0.15M MgCl(2), 0.1 M Tris-HCl; 2:1 ratio of SSB-Ct peptide:exonuclease U, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
