3C8S
Contributions of all 20 amino acids at site 96 to the stability and structure of T4 lysozyme
Replaces: 2NZNExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-07-04 |
| Detector | SDMS |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 61.000, 61.000, 97.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.560 - 1.680 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l63 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.290 |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.560 | 1.710 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.082 | |
| Number of reflections | 21406 | |
| Completeness [%] | 89.1 | 35.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.7 | 2 M NA/K PHOSPHATE PH 6.7 50 MM REDUCED BME 50 MM OXIDIZED BME, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 277K, pH 6.70 |
