3C8Q
Contribution of all 20 amino acids at site 96 to the stability and structure of T4 lysozyme
Replaces: 2NZBExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-01-01 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.960, 60.960, 97.460 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.640 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.18600 |
| Structure solution method | MOLECULAR SUBSTITUTION |
| Starting model (for MR) | ISOMORPHOUS WT L163.PDB |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.347 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | X-PLOR |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.630 |
| Rmerge | 0.080 |
| Number of reflections | 26629 |
| Completeness [%] | 99.0 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.7 | 2M NA/K PHOSPHATE PH 6.7, 50 MM OXIDIZED BME, 50 MM REDUCED BME, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 276K, pH 6.70 |
