3C8L

Crystal structure of a ftsz-like protein of unknown function (npun_r1471) from nostoc punctiforme pcc 73102 at 1.22 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-01-12
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837, 0.97978, 0.97964
Spacegroup name	C 2 2 21
Unit cell lengths	75.418, 78.628, 75.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.065 - 1.220
R-factor	0.159
Rwork	0.158
R-free	0.18400
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.746
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.065	44.065	1.260
High resolution limit [Å]	1.220	2.630	1.220
Rmerge	0.053	0.038	0.518
Number of reflections	65528	6668	5785
<I/σ(I)>	11.5	34.4	1.8
Completeness [%]	98.5	96.4	95.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	NANODROP, 2.0M (NH4)2SO4, 0.1M Tris-HCl pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K