3C7Z
T4 lysozyme mutant D89A/R96H at room temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-01-23 |
| Detector | SDMW |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.942, 60.942, 96.813 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 11.440 - 1.670 |
| R-factor | 0.187 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l63 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.078 |
| Data reduction software | SAN (DIEGO MULTIWIRE) |
| Data scaling software | SAN (DEIGO MULTIWIRE) |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 11.440 |
| High resolution limit [Å] | 1.670 |
| Rmerge | 0.059 |
| Number of reflections | 23622 |
| Completeness [%] | 95.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.7 | 2 M NA/K PHOSPHATE PH 6.7 550 MM NACL 50 MM REDUCED BME, 50 MM OXIDIZED BME , VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K, pH 6.70 |
