3C7V
Structural Insight into the Kinetics and Delta-Cp of interactions between TEM-1 Beta-Lactamase and BLIP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CAMD BEAMLINE GCPCC |
| Synchrotron site | CAMD |
| Beamline | GCPCC |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-05 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.3808 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.671, 129.044, 78.898 |
| Unit cell angles | 90.00, 91.20, 90.00 |
Refinement procedure
| Resolution | 35.090 - 2.070 |
| R-factor | 0.21563 |
| Rwork | 0.214 |
| R-free | 0.23846 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jtg |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.403 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.100 |
| High resolution limit [Å] | 2.070 |
| Rmerge | 0.084 |
| Number of reflections | 54728 |
| <I/σ(I)> | 13.1 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 298 | 12% PEG 8000, 0.1M Phosphate-Citrate, 0.1M NaCl, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
