3C7H
Crystal structure of glycoside hydrolase family 43 arabinoxylan arabinofuranohydrolase from Bacillus subtilis in complex with AXOS-4-0.5.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2007-07-08 |
Detector | PILATUS 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.770, 74.230, 107.340 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.650 - 2.000 |
R-factor | 0.15686 |
Rwork | 0.155 |
R-free | 0.19023 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.443 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.081 | 0.279 |
Number of reflections | 50933 | |
<I/σ(I)> | 19.9 | 6.1 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 7.7 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 4.0 M Na formate, 0.1 M betaine hydrochloride, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |