3C73
Structure of CEHC variant ResA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-14 |
| Detector | ADSC |
| Wavelength(s) | 1.06880 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.219, 60.169, 110.393 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.880 - 2.500 |
| R-factor | 0.18231 |
| Rwork | 0.178 |
| R-free | 0.25298 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.786 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.850 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 11418 | |
| <I/σ(I)> | 14 | 6.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.6 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 289 | 27% PEG 4000, 0.1 M tri-Sodium Citrate pH 5.6, 0.2M Ammonium acetate, 10 mM DTT. Protein concentration 12mg/ml in 20 mM MOPS pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
