3C70
HNL from Hevea brasiliensis to atomic resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-05 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8550 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 47.276, 106.344, 128.312 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 1.050 |
| Structure solution method | MR/rigid body placement |
| Starting model (for MR) | 1qj4 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.030 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC ((rigid body)) |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.060 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.045 | 0.303 |
| Number of reflections | 145202 | |
| <I/σ(I)> | 35 | 4.3 |
| Completeness [%] | 100.0 | 90 |
| Redundancy | 6.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 2% PEG400, 2M ammonium sulphate, 0.1M Hepes-Na, pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
