3C6Q
Apo and ligand-bound form of a thermophilic glucose/xylose binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.100, 75.750, 100.520 |
| Unit cell angles | 90.00, 89.90, 90.00 |
Refinement procedure
| Resolution | 19.868 - 2.300 |
| Rwork | 0.214 |
| R-free | 0.28100 |
| Starting model (for MR) | 2h3h |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.020 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.098 | 0.273 |
| Number of reflections | 53176 | 5743 |
| <I/σ(I)> | 11.86 | 4.3 |
| Completeness [%] | 97.9 | 89.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 290 | 0.1M HEPES, 23% PEG 10000, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
