3C5T
Crystal structure of the ligand-bound glucagon-like peptide-1 receptor extracellular domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-14 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 75.910, 75.910, 87.770 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.500 - 2.100 |
R-factor | 0.20503 |
Rwork | 0.203 |
R-free | 0.23809 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3C59 without the ligand present |
RMSD bond length | 0.019 |
RMSD bond angle | 1.702 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.3.0040) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.061 | 0.395 |
Number of reflections | 17273 | |
<I/σ(I)> | 27.1 | 7.3 |
Completeness [%] | 98.3 | 97.8 |
Redundancy | 10.9 | 11.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Tris-HCl pH 8.5, 0.1M MgCl2, 0.4M MgTartrate, 9mM n-Decyl-beta-D-thiomaltoside, VAPOR DIFFUSION, HANGING DROP, temperature 293K |