3C5R
Crystal Structure of the BARD1 Ankyrin Repeat Domain and Its Functional Consequences
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-04-02 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.847, 74.112, 51.174 |
| Unit cell angles | 90.00, 105.23, 90.00 |
Refinement procedure
| Resolution | 49.390 - 2.000 |
| R-factor | 0.2022 |
| Rwork | 0.199 |
| R-free | 0.26818 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1svx |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.227 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.680 | 1.830 |
| Number of reflections | 19640 | |
| <I/σ(I)> | 17.83 | 1.42 |
| Completeness [%] | 81.2 | 26.8 |
| Redundancy | 3.3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.2 Mg Chloride, 0.1 M Tris Hydrochloride pH 8.5, 25% w/v PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
