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3C5P

Crystal structure of BAS0735, a protein of unknown function from Bacillus anthracis str. Sterne

Experimental procedure
Experimental methodMAD
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 19-ID
Synchrotron siteAPS
Beamline19-ID
Temperature [K]100
Detector technologyCCD
Collection date2007-11-23
DetectorADSC QUANTUM 315
Wavelength(s)0.9793, 0.9795
Spacegroup nameC 2 2 21
Unit cell lengths132.915, 228.581, 132.093
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution46.850 - 2.900
R-factor0.178
Rwork0.175
R-free0.22600
Structure solution methodMAD
RMSD bond length0.016
RMSD bond angle1.560
Data reduction softwareHKL-3000
Data scaling softwareHKL-3000
Phasing softwareHKL-3000
Refinement softwareREFMAC (5.2.0019)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0003.000
High resolution limit [Å]2.9002.900
Number of reflections44927
<I/σ(I)>73.5
Completeness [%]99.999.8
Redundancy8.28.2
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP8.52893M NaCl, 0.1M Tris-HCl pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K

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