3C5P
Crystal structure of BAS0735, a protein of unknown function from Bacillus anthracis str. Sterne
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9793, 0.9795 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 132.915, 228.581, 132.093 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.850 - 2.900 |
R-factor | 0.178 |
Rwork | 0.175 |
R-free | 0.22600 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.560 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 44927 | |
<I/σ(I)> | 7 | 3.5 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 8.2 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 3M NaCl, 0.1M Tris-HCl pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |