3C5P
Crystal structure of BAS0735, a protein of unknown function from Bacillus anthracis str. Sterne
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793, 0.9795 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 132.915, 228.581, 132.093 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.850 - 2.900 |
| R-factor | 0.178 |
| Rwork | 0.175 |
| R-free | 0.22600 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.560 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 44927 | |
| <I/σ(I)> | 7 | 3.5 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 8.2 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 3M NaCl, 0.1M Tris-HCl pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
