3C5N
Structure of human TULP1 in complex with IP3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-04-11 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.97874 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.310, 83.630, 57.680 |
| Unit cell angles | 90.00, 94.01, 90.00 |
Refinement procedure
| Resolution | 23.702 - 1.800 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.22100 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.029 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 48246 | |
| <I/σ(I)> | 15.7 | 2.48 |
| Completeness [%] | 97.6 | 99.3 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 8000, 10 mM myo-inositol 1,4,5 trisphosphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
