3C58
Crystal structure of a complex between the wild-type lactococcus lactis Fpg (MutM) and a N7-Benzyl-Fapy-dG containing DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 91.356, 91.356, 140.943 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.690 - 1.900 |
| R-factor | 0.173 |
| Rwork | 0.172 |
| R-free | 0.20000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xc8 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.745 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.620 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.099 | 0.451 |
| Number of reflections | 47718 | |
| <I/σ(I)> | 17 | 3.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | HEPES, Sodium citrate, PH7.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | HEPES | ||
| 2 | 1 | 1 | Sodium citrate | ||
| 3 | 1 | 1 | HOH | ||
| 4 | 1 | 2 | HEPES | ||
| 5 | 1 | 2 | Sodium citrate | ||
| 6 | 1 | 2 | HOH |
