3C51

Crystal structure of G protein coupled receptor kinase 1 bound to ADP and magnesium chloride at 3.55A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-17
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	58.657, 92.527, 259.383
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.880 - 3.550
R-factor	0.28105
Rwork	0.281
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3c4z
RMSD bond length	0.008
RMSD bond angle	1.071
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	3.550
Number of reflections	17511

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.25	277	PEG 8000, NaBr, glycerol, MES pH 6.25, ADP pH 7.5, magnesium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 277K