3C4W
Crystal Structure of G protein coupled receptor kinase 1 bound to ATP and magnesium chloride at 2.7A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97967 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 202.889, 55.054, 122.699 |
| Unit cell angles | 90.00, 100.82, 90.00 |
Refinement procedure
| Resolution | 19.980 - 2.700 |
| R-factor | 0.19241 |
| Rwork | 0.192 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2acx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.331 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 37248 | |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.35 | 277 | PEG 3350, NaCl, glycerol, sodium tartarate, pH 4.35, ATP, magnesium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
