3C4A
Crystal structure of vioD hydroxylase in complex with FAD from Chromobacterium violaceum. Northeast Structural Genomics Consortium Target CvR158
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-01-18 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97900 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 89.179, 97.725, 40.605 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.880 - 2.300 |
R-factor | 0.202 |
Rwork | 0.202 |
R-free | 0.26200 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.105 | 0.473 |
Number of reflections | 30479 | |
<I/σ(I)> | 20.58 | 3.32 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.5 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | Protein solution: 10 mM Tris-HCl, 100 mM NaCl, 3 mM FAD, 5 mM DTT. Reservoir solution: 100 mM Bis-Tris pH 6.5, 25% PEG 3350, 200 mM MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 291K |