3C41

ABC protein ArtP in complex with AMP-PNP/Mg2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-29
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.95373
Spacegroup name	P 1 21 1
Unit cell lengths	48.277, 82.921, 68.630
Unit cell angles	90.00, 103.90, 90.00

Refinement procedure

Resolution	50.000 - 2.250
Rwork	0.224
R-free	0.27700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2olj
RMSD bond length	0.007
RMSD bond angle	1.470
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.072	0.380
Number of reflections	21590
<I/σ(I)>	16.7	2.5
Completeness [%]	87.2	66.7
Redundancy	3.6	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	26% PEG3350, 0.1M Na-Acetate, 5% Isopropanol, 0.01M MgCl2, pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K