3C3Z
Crystal structure of HIV-1 subtype F DIS extended duplex RNA bound to ribostamycin
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2006-05-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9788, 0.9793, 0.9732 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.670, 30.530, 57.680 |
| Unit cell angles | 90.00, 121.31, 90.00 |
Refinement procedure
| Resolution | 18.440 - 1.500 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.22700 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.055 | |
| Number of reflections | 46246 | |
| <I/σ(I)> | 27.9 | 5.9 |
| Completeness [%] | 98.6 | 98.5 |
| Redundancy | 9.2 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 310 | 300mM KCl, 2mM MgCl2, 50mM Na Cacodylate, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 310K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Na cacodylate | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | KCl | ||
| 4 | 1 | 2 | Na cacodylate | ||
| 5 | 1 | 2 | MgCl2 | ||
| 6 | 1 | 2 | KCl |
