3C3T
Role of a Glutamate Bridge Spanning the Dimeric Interface of Human Manganese Superoxide Dismutase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 81.293, 81.293, 241.693 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.970 - 2.200 |
| R-factor | 0.175 |
| Rwork | 0.175 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1luv |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.230 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 25122 | |
| <I/σ(I)> | 33 | |
| Completeness [%] | 94.1 | 68.3 |
| Redundancy | 5.6 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 300 | 100mM Imidazole, 100mM Malic Acid, 3.0M Ammonium Sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
