3C3N
Crystal structure of dihydroorotate dehydrogenase from Trypanosoma cruzi strain Y
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-07 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.427 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 82.390, 123.920, 128.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.800 - 2.200 |
| R-factor | 0.207 |
| Rwork | 0.206 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.800 | 2.230 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.101 | 0.323 |
| Number of reflections | 62549 | 2062 |
| <I/σ(I)> | 9.72 | 4.3 |
| Completeness [%] | 96.6 | 77.2 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 1.5M ammonium sulfate, 5% v/v 2-Propanol, 0.1M sodium acetate, pH 5.0, 2mM Dithiothreitol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
