3C39
Crystal Structure of human phosphoglycerate kinase bound to 3-phosphoglycerate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.616, 55.790, 93.230 |
| Unit cell angles | 79.18, 84.33, 83.23 |
Refinement procedure
| Resolution | 50.100 - 1.850 |
| R-factor | 0.19383 |
| Rwork | 0.191 |
| R-free | 0.27281 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vjc |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.905 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.4.0062) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.290 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.048 | 0.340 |
| Number of reflections | 44499 | |
| <I/σ(I)> | 14 | 1.9 |
| Completeness [%] | 77.1 | 50.4 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 291 | 2.6M NaKPO4, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
