3C2Q
Crystal structure of conserved putative LOR/SDH protein from Methanococcus maripaludis S2
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-21 |
| Detector | SBC-3 |
| Wavelength(s) | 0.979229 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 119.592, 119.592, 161.737 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 96.230 - 2.000 |
| R-factor | 0.19215 |
| Rwork | 0.191 |
| R-free | 0.21694 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.479 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.056 | 0.252 |
| Number of reflections | 75521 | |
| <I/σ(I)> | 6.2 | |
| Completeness [%] | 93.3 | 99.7 |
| Redundancy | 7.4 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 1.00M Sodium citrate, 0.10M Imidazole pH 8.0, 0.05M Sodium fluoride, cryoprotected with 25% v/v Glycerol and saturated Sodium citrate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
