3C2B
Crystal structure of TetR transcriptional regulator from Agrobacterium tumefaciens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.134, 72.791, 127.198 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.800 - 2.100 |
| R-factor | 0.2006 |
| Rwork | 0.198 |
| R-free | 0.24060 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.486 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.800 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.081 | 0.580 |
| Number of reflections | 23127 | |
| <I/σ(I)> | 10.5 | 1.86 |
| Completeness [%] | 93.6 | 60.4 |
| Redundancy | 8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10.5 | 294 | 0.1 M CAPSO buffer, 20% PEG 2000 MME, 0.3 M NDSB-195, pH 10.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
