3C2A
Antibody Fab fragment 447-52D in complex with UG1033 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-25 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.251, 76.482, 114.130 |
| Unit cell angles | 90.00, 101.49, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.2413 |
| Rwork | 0.240 |
| R-free | 0.29775 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q1j |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.637 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 60586 | |
| <I/σ(I)> | 27.1 | 4 |
| Completeness [%] | 85.7 | 86 |
| Redundancy | 5.1 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.9 | 298 | 1.58M ammonium sulfate 0.1M Tris 1mM CuCl2, pH 7.9, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
