3C26

Crystal structure of a putative acetyltransferase (NP_394282.1) from Thermoplasma acidophilum at 2.00 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-12-09
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97895
Spacegroup name	P 43 21 2
Unit cell lengths	86.820, 86.820, 67.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.488 - 2.000
R-factor	0.182
Rwork	0.179
R-free	0.24200
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.609
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.4.0067)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.488	29.488	2.070
High resolution limit [Å]	2.000	4.300	2.000
Rmerge	0.105	0.032	0.600
Number of reflections	17927	3338	3221
<I/σ(I)>	10.53	33.1	2.2
Completeness [%]	99.8	98.9	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	293	NANODROP, 0.2M NaNO3, 20.0% PEG 3350, No Buffer pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K