3C1Z
Structure of the ligand-free form of a bacterial DNA damage sensor protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.006 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 108.930, 108.930, 166.470 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
Rwork | 0.222 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3c1y |
RMSD bond length | 0.006 |
RMSD bond angle | 1.108 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.500 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.074 | 0.425 |
Number of reflections | 45103 | |
<I/σ(I)> | 12.14 | 2.72 |
Completeness [%] | 99.5 | 98.6 |
Redundancy | 4 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M Tris pH 7.5, 0.2M Ammoniumacetate, 35% MPD, VAPOR DIFFUSION, SITTING DROP, temperature 293K |