3C1E
Crystal structure of Staphylococcal nuclease variant Delta+PHS L125K at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2008-01-10 |
| Detector | BRUKER APEX II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.075, 60.453, 38.144 |
| Unit cell angles | 90.00, 93.10, 90.00 |
Refinement procedure
| Resolution | 27.610 - 1.900 |
| R-factor | 0.201 |
| Rwork | 0.195 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1TQO.PDB |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.434 |
| Data reduction software | SAINT |
| Data scaling software | SAINT (V7.34A) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.090 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.036 | 0.171 |
| Number of reflections | 11202 | |
| <I/σ(I)> | 38.45 | 6.05 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 11.09 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 32% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 9.0, vapor diffusion, hanging drop, temperature 277K |
