3C19
Crystal structure of protein MK0293 from Methanopyrus kandleri AV19
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2007-10-17 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9796 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 108.456, 108.456, 108.456 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.24925 |
| Rwork | 0.249 |
| R-free | 0.26905 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.252 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.072 | 0.490 |
| Number of reflections | 7516 | |
| <I/σ(I)> | 6.8 | 1.8 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 15.2 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 100mM Tris-HCl pH 8.5, 1.45M Ammonium dihydrogen phosphate, 10% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
