3C0V
Crystal structure of cytokinin-specific binding protein in complex with cytokinin and Ta6Br12
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-23 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.2547, 1.2580, 1.2703 |
| Spacegroup name | P 64 |
| Unit cell lengths | 113.219, 113.219, 85.077 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.310 - 1.800 |
| R-factor | 0.15897 |
| Rwork | 0.158 |
| R-free | 0.20729 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.760 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.033 | 0.391 |
| Number of reflections | 56873 | |
| <I/σ(I)> | 55.1 | 5.5 |
| Completeness [%] | 99.0 | 97.8 |
| Redundancy | 7.4 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.4M SODIUM CITRATE, 0.1M HEPES, pH 7.5,3mM ZEATIN, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
