3C0U
Crystal structure of E.coli yaeQ protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97948 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 127.277, 127.277, 72.059 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.680 - 2.700 |
| R-factor | 0.21079 |
| Rwork | 0.210 |
| R-free | 0.23420 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.230 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.085 | 0.596 |
| Number of reflections | 16728 | |
| <I/σ(I)> | 55.36 | 2.64 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 15.7 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 0.2M Ammonium sulfate, 0.1M Sodium cacodylate pH 6.5, 30% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
