3C0R

Structure of Ovarian Tumor (OTU) domain in complex with Ubiquitin

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-25
Detector	MARRESEARCH
Wavelength(s)	0.9793, 0.9795, 0.9566
Spacegroup name	P 64
Unit cell lengths	107.311, 107.311, 100.239
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.626 - 2.315
R-factor	0.198
Rwork	0.196
R-free	0.24300
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.230
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.626	2.390
High resolution limit [Å]	2.315	2.315
Rmerge		0.357
Number of reflections	28554
<I/σ(I)>	30.2	3.29
Completeness [%]	99.8
Redundancy	18.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	6.5	277	MES, ammonium acetate, PEG3350, pH 6.5, hanging drop, temperature 277K