3C0Q
UVDE E175A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-16 |
| Detector | MAR225 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 113.380, 113.380, 89.080 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.030 - 2.740 |
| R-factor | 0.20034 |
| Rwork | 0.198 |
| R-free | 0.24510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bzg |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.318 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.030 | 2.890 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Rmerge | 0.087 | 0.364 |
| Number of reflections | 9213 | |
| <I/σ(I)> | 8 | |
| Completeness [%] | 99.3 | 99.8 |
| Redundancy | 4.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | 0.5M ammonium sulfate, 0.1M acetate, pH6.0, 1M lithium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
